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                      Astaxanthin for Feed, Technical Bulletin, January 2007

    ASTAXANTHIN PARTNERS LTD

                                                                                                                   Technical Bulletin

Aquasta ®

DESCRIPTION

Aquasta® is Astaxanthin Partners Ltd's trade name for its inactivated dried yeast product made from the organism Phaffia rhodozyma. Aquasta® provides a natural source of the pigment astaxanthin, which imparts the characteristic red color to the flesh of salmon, trout, and other aquatic species. Astaxanthin Partners Ltd's yeast is specially formulated to maximize bioavailability of the pigment.

APPLICATION

Aquasta® is a free-flowing granular powder that can be readily blended with other feed ingredients to meet formulator's specifications.   Aquasta® is available with different concentrations of astaxanthin and this has to be considered to calculate the appropriate inclusion rate.  For example, if the astaxanthin content is 10,000ppm, and the target is to obtain 60 mg/kg astaxanthin in the feed, 6 kg of Aquasta® should be incorporated per tonne of feed.

This product is for use as a coloring agent in fish feeds at a rate not to exceed 100 mg/kg astaxanthin in the feed.

TYPICAL ANALYSIS

Description                          Amount (%)

Moisture (vacuum oven)                 3.03

Total Carbohydrate                          40.5

    Beta glucans                                  16.1

    Mannans                                        10.5

Protein (N x 6.25)                               17.4

Phosphorus                                       0.24

Fat (acid hydrolysis)                        37.9

Ash (acid hydrolysis)                       1.11

Estimated Caloric Value                    573 Calories/100g

Astaxanthin                                       Minimum 8,000 ppm

Disrupted yeast                                > 85 %

(measured by extractability in acetone)

Total CFU                                         NMT 10,000/g

E. Coli                                                                    0/g

Salmonella                                                            0/g

Total Fungi                                            NMT 100/g

Vitamins:                                                                                 Minerals:

Vitamin A                                1,030 I.U./100g                      Calcium                               22.9 mg/100g

Thiamine                                      2.3 mg/100g                       Iron                                       4.4 mg/100g

Riboflavin                                   0.91mg/100g                       Magnesium                        35.5 mg/100g

Pantothenate                             0.75 mg/100g                       Potassium                           155 mg/100g

Niacin                                           2.6 mg/100g

B6 (pyridoxine)                         0.31 mg/100g

Biotin                                        0.026 mg/100g                       Arsenic                               < 0.6 ppm

Folic Acid                                  0.69 mg/100g                       Cadmium                             < 0.1 ppm

Choline                                       328 mg/100g                        Lead                                    < 0.2 ppm

Vitamin C                                    250 mg/100g                        Mercury                           < 0.02 ppm

Fatty Acids:              Amount (%) (reported as a percentage of the total identifiable fatty acids present)

C8-0                           < 0.1

C10-0                         < 0.1

C13-0                            0.6

C12-0                         < 0.1

C14-0                            0.6

C14-1                         < 0.1

C15-0                            0.4

C16-0                          21.2

C17-0                            0.5

C18-0                            2.9

C18-1                          25.8

C18-2                          42.1

C18-3                            2.1

C19-0                            0.4

C20-0                            0.5

C20-1                         < 0.1

C20-4                         < 0.1

C20-5                         < 0.1

C21-0                            0.1

C22-0                            0.4

C22-1                         < 0.1

C22-5                            0.9

C22-6                         < 0.1

C24-0                            0.7

C24-1                         < 0.1

Amino Acids:   Amount (%)         (crude protein basis)

Aspartic Acid            7.2

Threonine                   4.0

Serine                          4.0

Glutamic Acid            8.8
Proline                         3.3

Glycine                        3.9

Alanine                       2.1

Valine                          4.7

Isoleucine                   3.8

Leucine                       6.6

Tyrosine                     2.5

Phenylalanine            3.6

Histidine                     1.9

Lysine                         4.6

Arginine                     4.0

PACKAGING

Aquasta® is packaged in 10 and 20 KG boxes contained in a heat-sealed foil bag.

STORAGE AND HANDLING

Shelf life 12 months when unopened packages are stored at or below 40 degrees C.   Avoid unnecessary exposure to air and light.   Refrigeration preferred.

DESCRIPTION

Inactive dried yeast product.

MANUFACTURED FOR:                        MANUFACTURED BY:

Astaxanthin Partners Ltd.                                          Tate & Lyle Citric Acid

2200 E. Eldorado St.                                                     Denison Road, Selby

Decatur, IL 62525                                     North Yorkshire Y08 8EF

USA                                                          ENGLAND

1-217-423-4411                                                              44 (0) 1757-703691

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